Work Packages
The project integrates microphysiological systems, advanced cytometry techniques, and data-driven analysis to evaluate drug efficacy in a physiologically relevant environment.
WP1 – Organ-on-Chip Platform
Description
This work package focuses on the development of a microphysiological system, MPS, reproducing the perivascular niche of bone tissue, a key site for cancer metastasis.
Objectives
1. Reproduce the bone microenvironment in vitro
2. Mimic physiological fluid shear stress conditions
3. Enable controlled administration of therapeutic agents
Main Activities
1. Design and fabrication of the organ-on-chip platform
2. Cell culture of bone and cancer cells
3. Experimental validation under dynamic conditions
WP2 – IODC Platform Development
Description
This work package aims to develop an impedance optical-deformability cytometry (IODC) module for high-throughput characterization of cancer cells.
Objectives
1. Probe electro-mechanical properties of cells 2. Enable high-throughput measurements
3. Integrate with the organ-on-chip system
Main Activities
1. Design and fabrication of the IODC device
2. System integration and calibration
3. Performance testing
WP3 – Data Acquisition
Description
This work package is dedicated to collecting multimodal experimental data from the developed platforms.
Objectives
1. Acquire high-quality time-lapse microscopy data
2. Collect IODC measurements
3. Ensure reproducibility of experiments
Main Activities
1. Time-lapse microscopy (TLM) video acquisition
2. High-throughput IODC measurements
WP4 – Data Analysis and Integration
This work package focuses on advanced data processing and integration of multimodal signals to evaluate therapy efficacy.
Objectives
1. Develop robust data analysis pipelines
2. Combine TLM and IODC signals
3. Achieve high prediction accuracy
Main Activities
1. Signal processing and feature extraction
2. Multimodal data integration
3. Development of interpretable models
This project has received funding from the European Union’s Framework Programme for Research and Innovation Horizon Europe (2021-2027) under the Marie Skłodowska-Curie Grant Agreement No. 101202231.
